Journal: Frontiers in Genetics

Article Title: Distinct Associations of BMI and Fatty Acids With DNA Methylation in Fasting and Postprandial States in Men

doi: 10.3389/fgene.2021.665769

Figure Lengend Snippet: DNA methylation trend in fasting and postprandial states across BMI classes. Open squares and solid circles, fasting and postprandial state, respectively. Gene name and EPIC array Ow-dmCpG ID are indicated above each graph. N, Ow, and Ob, normal weight, overweight and obese, respectively.

Article Snippet: Ow-dmCpG genomic distribution relative to gene compartments or CpG islands was significantly skewed in favor of promoters and first exons (∼1.8-fold enrichment, p = 0.006; Chi-square test) and CpG islands (∼2.3-fold, p = 9.7 × 10 –8 ), where enrichment was highest of any dmCpG set, compared to the distribution of the 736,741 Infinium methylation EPIC array reference probes ( ).

Techniques: DNA Methylation Assay

Journal: Scientific Reports

Article Title: Endoplasmic reticulum protein 29 (ERp29) confers radioresistance through the DNA repair gene, O 6 -methylguanine DNA-methyltransferase, in breast cancer cells

doi: 10.1038/srep14723

Figure Lengend Snippet: ( a ) ERp29 expression promoted/inhibited promoter demethylation of tumour suppressors/pro-oncogenes identified by Methylation PCR arrays. ( b ) Tumour suppressor genes CDH1 and MGMT were transcriptionally activated by ERp29. The mRNA and protein expressions were examined by RT-PCR and Western blot. ( c ) MS-PCR analysis for MGMT promoter methylation/demethylation. Note that the ratio of demethylation/methylation was highly increased in the ERp29-transfected cells (clone B and E). Cells treated with 5′-aza-dC was used as a positive control for demethylation. Genomic DNA was extracted and converted with sodium bisulfite. MS-PCR was performed as described in “Materials and Methods”. **p < 0.01 versus control.

Article Snippet: Promoter methylation/demethylation was performed using the Methyl-Profiler™ DNA Methylation PCR Array (SuperArray Bioscience Corporation, Frederick, MD).

Techniques: Expressing, Methylation, Reverse Transcription Polymerase Chain Reaction, Western Blot, Transfection, Positive Control

Journal: Scientific Reports

Article Title: Endoplasmic reticulum protein 29 (ERp29) confers radioresistance through the DNA repair gene, O 6 -methylguanine DNA-methyltransferase, in breast cancer cells

doi: 10.1038/srep14723

Figure Lengend Snippet: ( a ) ERp29 expression decreased the level of DNMT1 whereas ERp29 knockdown upregulated the expression of DNMT1. The expression of DNMT3A or 3B was not markedly affected by ERp29. *p < 0.05, **p < 0.01, relative mock-transfected control or controL siRNA. ( b ) Reduction of DNMT1 by siRNA upregulated MGMT expression in MDA-MB-231 cells. MDA-MB-231 cells were transiently transfected with control siRNA or DNMT1 siRNA (#1) for 48hours and the expression of DNMT1 and MGMT was examined. ( c ) MGMT promoter methylation/demethylation. Genomic DNA was extracted from the MDA-MB-231 cells transfected with control siRNA or DNMT1 siRNA and the MS-PCR was done as described in “Materials and Methods”. **p < 0.01, ***p < 0.001, versus control.

Article Snippet: Promoter methylation/demethylation was performed using the Methyl-Profiler™ DNA Methylation PCR Array (SuperArray Bioscience Corporation, Frederick, MD).

Techniques: Expressing, Transfection, Methylation